Today

today was actually quite a normal day, but i feel like blogging abt some minor things that happened today.

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during work today, i was rinsing my 2L (very long one) measuring cylinder at the washing sink. my colleague was standing behind me waiting to wash her apparatus. so i kindly stepped back to let her use the sink. the measuring cylinder was still my hands. so when i felt that the rinsing was almost done, i poured the water from the cylinder into the sink. i dunno whether the water had splashed onto her or wat she's just shocked that i suddenly poured water near her, she looked at me for a brief moment. i din feel anything was wrong, i just carried on with what i was doing. and then i realised, for the whole of today, she nv really smile at me liaoz... (she usually has a polite smile on her face)

but as i was somebody who gets pissed easily with other ppl, i'm qt sensitive to wat can make ppl pissed. haiz. i dun wan to make enemies in an unfamiliar place, esp. when i'm really really a very small fry. haiz. i tink i better buy nice cookies for them on my last day of internship, so that my FYP will have 好日子过...

and anw, just when i tot i could leave to meet Erina to pass her my GOOD sleeping bag, I went to check on my Nu-PAGE gel running on electrophoresis. my sup was at a meeting and said that she could take over from there. but just when i was about to leave, she was back. and i had to continue doing the transferring of gel (she went for other short meeting after she came back to ask me to continue)

so after i finished the transfer, it was already 6pm. walked to the bus stop and waited for 25min for 95 to meet Erina. when i met her, it was already 6.30pm and i had to reach city hall at 7pm. had arranged with Xinhui (HCJC) to meet her in school after her lab too, to go tog to city hall. so being the "clever" me, i told her 95 and MRT would be very crowded and 95 also had a very long frequency. we went to take 197 instead...

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and we reached City Hall at 7.45pm... when we arrived at Muthu's Curry for "jiemeiz meet Xiaoyang" session, it was already like 8pm. Muthu's Curry is okay... but the service is quite... haha... u infer from wat i've to say about the "Can you refill our ice water?" incident. we actually asked for ice water 4 times and it's only the 4th time when we actually got our ice water refilled. we figured that they need to get water from the tap, freeze it down, and take it out, so it's taking such a long time. hahaha..

planned to eat Gelare's 50% (only on Tuesday) waffles but walked past "Donut Factory" and Wei Ting decided to try it. So all of us bought 1 piece each with her too. i had "mint dark chocolate". it was okay, quite soft and chocolatey. but all of them said "Krispy Kreme" one was nicer... and i was the only one dere who hasn't tried it b4.. sianz.. haha..

tok cok abit about old bandmates. xiaoyang is still the same. i realised all of those who went overseas grew thinner. they said it was due to their money saving plans. hahaha

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finally can choose projects liaoz. but i would most probably end up with the BTI one. dun wan to waste my 3 mths here haha... this is the project title:

Role of Epithelial Cell Adhesion Molecule (EpCAM) in Maintaining Proliferation of Human Embryonic Stem Cells

sounds chim? i tink so too haha!

NUSWS BBQ outing

had a bbq outing with nusws. haha. kind of initiated it, with the idea of a mini-DND. it turned out well. with 28 ppl attending. :)

food was ok. hmm felt abit cheated by EZB though. but, not bad la, for its convenience.

thanks danK for ordering the food and booking the pit. thanks erina for counting the money. thanks nick for helping to drive around the food and publicise/jio ppl. thanks everybody for attending YAY~~

ermz, b4 the bbq, went to cycle. i think pasir ris park is a better place to cycle on weekends than ECP due to its light traffic. BUT STILL I DUN UNDERSTAND Y THESE PLACES HAVE TO BE IN THE EAST! hmmpf!

oh and nick's new speakers are quite cool. at least our bbq was not too quiet. got nice band music as accompaniment hehe.

ok i need to:
1. change strings
2. get books for my student
3. start my report
4. read and UNDERSTAND my journal
5. upload more pictures on Friendster.

haha.. not now. hehe time to slp :)

Shagged day!

i worked almost every second during my attachment working hours today. pls skip the scientific details if u dun wan to read :P

Morning:

continued doing my Western blot, adding primary antibody, washings, secondary antibody, washings, develop films in dark room... addition of horse radish peroxidase got error (due to lack of skills OOPS) redo addition of HRP, develop films again.

observed killing of cells and fixing them, before doing FACS (fluoresence activated cytometric sorting)... and it was 3pm by then...

had initially wanted to go with the rest to eat at Clementi, in the end need them to da bao the beef noodles for me. and it was really good. so lunch was at 3pm in the pantry, eating ta bao-ed beef noodles plus my leftover lipton tea. then, cant even have a full 1 h of lunch, because i had a timer on for one of the washings and had to return to lab after 30min of "lunch". SAD and shagged...

Afternoon:

continued western blot, did the blot with alkaline phosphatase this time, instead of HRP...
helped in extraction of RNA

BLEH... and it was already time to go to practise at CFA.

I realise i miss playing with a section :(

Water spout + VJC concert

Last friday, there was a commotion at my workplace, when somebody spotted a "twister-like" cloud while she was drinking water and gazing out of the window. and seconds later, u find the office with excited researchers toking abt it and suddenly running out of the office to catch a better view at the "Sky Bridge" at Level 5. they were even saying things like "if the crane thing fell when the twister is near it, we noe it's time to run" and someone even wanted to report to Straits Times.

and from the sky bridge, i took this picture with my camera phone. not very clear. but at least u can see sth.

after a like 10min of toking abt twister and it sweeping across the city from swissotel, i left the excited group when they said that it had subsided. HAHA.

actually, to me, the whole commotion was quite a comic-relief and an excuse to stop work for a while. heh. how often do u see ur office of researchers running around with video cameras and cameras. HAHA


Later that night, after VJC concert, Cloud showed me this link : Channelnewsasia article.
So it's a water spout, not a twister. HAHA

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VJC concert

i love 3rd Symphony. hehe.. emotionally draining. tho VJC din play very well (due to lack of rehearsals) i think 3rd movt was quite "emotionally delivered". 4th movement was messier because it's a more technical and fast movt. i love their Spartacus too! hehe. Concertino for bass tuba was ok, in terms of tone, it was quite impressive, but the soloist needs to work on projection.

then after the concert, went for supper at macs. den those west and north ppl had to leave earlier than the east ppl to catch the last train home.

More updates!

things are getting settled, mainly by acting as a grumpy customer. It really works if u are thick-skinned enough to complain and show ur dissatisfaction.

• in my last last entry i was talking abt the 4 emails i sent to the helpdesk personnel about my work login. in that email, i said sth like "i'm quite upset at this because of a lack of immediate assistance for something that had affected me so much" ... then on Monday morning, the IT guy came to my office and helped me fix it. u noe wat's the prob? my login name "chiahs" is now "chiaha"... eeeeeks.. cos they set it wrongly.... so i'm Hui A___... HAHA [but now i sense a little problem when using one of the lousier comp. shall see how tmr...]
• the STA travel card. emailed the NUS branch instead of the headquarters and the person responded by saying she mailed out already, but nobody seems to want to take the responsibility. she said that the mailing thing is very prone to become missing. then again, if u're offering the mail service, cant the customer use it? and do they really think that the customer will noe that the STA mailing service will be very prone to errors? so after a few grumpy replies asking about replacement card, the person asked me to get the card tmr... bleah... so sometimes, things can be done, but ppl are juz plain lazy or are just too proud to take up the responsibility (i guess by correcting it, u're admitting that u're in the wrong? haiz watever...)
• glad that i've a group of batch coordinators to help me with getting members for alumni band :) hope attendance will be better on Saturday.

something quite disturbing (not really scary but juz... not very nice....) happened. Some frenz whom I chat with almost everyday suddenly disappear, only to find out that they went overseas... without telling everyone else.... (i'm like ... huh?...) I noe it's supposed to be some secret, but.... sometimes things are not v nice lidat... [somewhat feel that the "family" thing is creating an insider-outsider effect]... i shan't go on.