BCM-ed finally

not very impressive leh. hahaah. maybe really have to try a 2nd and 3rd time to get addicted to it. haha :P

some overdued random pics from the holidays:

ARX bday. duno y only chris is in it. haha

Weixin said "你会不会觉得那个画面很满？" :P

SPIZZA~~!

and SPIZZA~~. this is ultimate. the egg damn messy after that.

the biological safety cabinet which i do my cell work at.

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a chilly true story which i heard from my mum today. duno whether it's correct to post here, but it disturbed me for the whole bus ride when we were going home.

my mum said she had a "colleague" who worked in the same building (colleague as in different companies but same building). she was pregnant but came to office one day and her babies (ultrasound showed twins) were gone. she was crying. and the only thing that happened was that she dreamt that she delivered. and then, it was gone when she woke up. the doctor cannot give her an explanation too. what he could do was just to gif her a week of MC.

it's damn sad and disturbing.

Crazy day (and more to come) + chinese physician visit

Warning: alot of scientific terms. ignore if u wan.

8.20am: reached lab, prepared real-time PCR plates (booked machine at 1pm)
9.15am: harvest cells, split Cos-7 cells
10.30am: lyse cells to get proteins, start incubating plates for MTS assay
11.30am: start DC protein assay for proteins
12.30pm: calculate concentration of proteins, start Western blotting proteins boiling loading of gels (4 15-well gels!), 1st time point for MTS assay
1pm: put in 1st plate for real-time PCR
1.30pm: hungry, hypoglycemic, giddy. wanted to endure till running gels. still loading gels.
2pm: BTH. zipped off to 7-11 to get sandwich. had a 15min rest in the pantry while drinking milo and eating the sandwich, waiting for glucose level to rise.
2.30pm: continued loading of gel, 2nd time-point for MTS assay
3.30pm: run gel, make transfer buffer, analyse MTS assay results, get plate from real-time PCR, put in 2nd plate. analyse results.
6pm: preparing for transfer of gels to membranes. make milk (not for drinking one, it's for the membranes!), take out plate from real-time. analyse results for real-time, do abit of writing, check emails/facebook
7pm: blocking of membranes in milk, make enuf antibodies, cont writing and facebooking/emails
8pm: washing of membranes
8.25pm: incubate membranes with antibodies
8.30pm: HOME! arranged to meet LC (who's also gg home from lab) on the bus :P

i duno wat kept me going today.

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anw visited the chinese physician yest for my cough:

“哇，你要少吃木瓜，橙，萝卜，你的手掌很黄。。。你的肝功能不好，很容易发热生病。。。你开始有背酸了对不对，手上显示出来了。。。你的肠胃也不好。。。最近睡得不好是不是？“

and that's above my cough condition...

*sweats*

alright not am i noe i

with loads of things to worry and think about, it's hard to feel good. watching the scenery whiz past me in the bus with music blasting into my ears, i paid no attention to anything. i noe i'm not alright.

to add on the the stress & depression, i'm still sick and cant exercise as an outlet to "emit" all these. i want to badminton and run. band badminton sessions are not for me, cos i'll be trapped in lab and seminar. runs are too strenuous for now. (evening badminton, anyone?)

hard to find someone to face a moody me, i engage in a new hobby called sleep, and not wanting to wake up. and of course, i have to wake up. to go to some place u noe where. (it's the title of this blog)

i wan to go to the zoo with some nonsense frenz... to laugh at the hippopotamus. holidays are ending. :(

haiz.

Dying in there

I'm rather upset by these 2 weeks of lab results.

When my Western blot came up with nice bands 2 days ago, i thought my luck had reversed. but subsequent proteins cant be detected anymore. i'm damn lost at what to do next. what i'm doing now is only to redo the whole cycle, again and again. cos i duno what went wrong, cos by right should have.

e.g. i'm testing for cell cycle, thus, detecting for cyclins. HOW CAN I NOT DETECT ANY CYCLINS IN MY CELLS?? like cells should ALL be expressing cyclins, just to different extent.

okiez tt's the summary.

feeling down after i "finished" what i'm supposed to do today at lab. i wanted to sms LC to 倾诉 but in the end, decided not to. i've been repeating my things to ppl who've been asking me "how's ur FYP" and and then i got suggestions which i've already tried or i noe wun work. it's really good to here that frenz are still there trying to encourage me and telling me to do this and that but sometimes, i dun feel like hearing those anymore.

i just want my sup to come back.

anyway i've arranged a meeting with her on Monday 2pm when she's back in the lab. I'm really looking forward to this meeting. at least then, i really have somebody who knows wat to do and wat went wrong, hopefully. or at least try to gear my project towards something which can be done within 1 more month.

can somebody take over my FYP and airmail me to ermz... a small little cottage in... Hokkaido?

woah.

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anyway i've been down with cough and cold. my cold was "cured" by antibiotics which again I went to the same GP who never fails to give me antibiotics. haha! (i'm the 33% of patient population who loves antibiotics) but at least i noe it's a relapse and it should be bacterial and so antibiotics is the suitable therapy wad.

and my cough since young, can never be cured fully by western med. so i heeded Peiting's father's advice to buy 夏桑菊 from the Medical Hall. It works quite well. but have to really drink faithfully, cos TCM effect is quite slow to kick in. it's quite cheap, $1.40 for 20 sachets. and i'm drinking around 3 sachets per day. still coughing, but not to the extent of coughing my lungs out already.

i think I quite like the combination of Western and Chinese medicine. in a way, the Western antibiotics works to eliminate the infection but the TCM kicks in to equilibrate the internal balance (pharmers, still rmb the 五脏六腑 PP3 presentations? =P ). haha

Kimage Promotion

I think all of u shd know this:


and so i went.

dye + rebond + rebond treatment + dye treatment + haircut = $338

not too bad with so many things done.
if u wan, can get the member card from me. :)

bad news: my hairstylist (Xavier) is away for 6 months cos he went to upgrade his skills. haa. today got this lady who looks quite grumpy but super 细心 in her work. not bad! but got this guy (newbie) called Kelvin who helped with the drying of hair and "ironing" of hair. painful sia~~

i've tried... not once, not twice.. but many times. this time i thought and hope that things would become more friendly, but it backfired.

but i'm not going to be sad anymore. it's not worth it.

2008

Hello!

it's a new year! I think ppl start making new year resolutions because the past year hasn't been satistory. for me, i have never seriously made any new year resolutions for the past 22 years but now, by putting it on my blog, i hope it'll help. haha.

In 2007, I was not as happy as I seem. It had been a tough year, in every aspect.

So in 2008, I'm going to:
1. Stop dreaming, start living (sounds BIG. but start with a general idea first)
2. Be happy in whatever I'm doing, even if it's cleaning the toilet. applies alot to lab work and FYP. e.g. I should keep smiling in lab. (no, i'm not gg to do that example. just cant think of a concrete example)
3, Find what I want in life (since i'm going to start work soon)
4. More time for family. Do more things for parents. Help out in family things more. e.g. buy CNY stuff without letting them buy more than 50%. and be present for ALL family outings, unless really bo bian.
5. Be more understanding and tolerant. Dun be impulsive. e.g. if friends are in a bad mood, dun be upset but try to understand what happened and empathise. dun throw a tantrum before first analysing the situation.
6. Exercise consistently like 3times a week or MINIMUM once a week. (bleah. i'll try)
7. Mug hard not for CAP but for those who need my help in the future. (sounds BIG too!). basically to still mug and be happy. e.g. to study and constantly trying to link to future work.
8. Despite busy schedule, still find it a joy to help others. e.g. dun say "i'm too busy to help" unless i really mean it. e.g. take part in at least 3 sets of voluntary work this year.
9. anything else?

anyway, it has been good food throughout 31dec and 1jan. haha! will blog more if i wan to post the pics.

to all my friends, HAPPY NEW YEAR. i hope all of u find joy in the new year!