8.20am: reached lab, prepared real-time PCR plates (booked machine at 1pm)
9.15am: harvest cells, split Cos-7 cells
10.30am: lyse cells to get proteins, start incubating plates for MTS assay
11.30am: start DC protein assay for proteins
12.30pm: calculate concentration of proteins, start Western blotting proteins boiling loading of gels (4 15-well gels!), 1st time point for MTS assay
1pm: put in 1st plate for real-time PCR
1.30pm: hungry, hypoglycemic, giddy. wanted to endure till running gels. still loading gels.
2pm: BTH. zipped off to 7-11 to get sandwich. had a 15min rest in the pantry while drinking milo and eating the sandwich, waiting for glucose level to rise.
2.30pm: continued loading of gel, 2nd time-point for MTS assay
3.30pm: run gel, make transfer buffer, analyse MTS assay results, get plate from real-time PCR, put in 2nd plate. analyse results.
6pm: preparing for transfer of gels to membranes. make milk (not for drinking one, it's for the membranes!), take out plate from real-time. analyse results for real-time, do abit of writing, check emails/facebook
7pm: blocking of membranes in milk, make enuf antibodies, cont writing and facebooking/emails
8pm: washing of membranes
8.25pm: incubate membranes with antibodies
8.30pm: HOME! arranged to meet LC (who's also gg home from lab) on the bus :P
i duno wat kept me going today.
================================
anw visited the chinese physician yest for my cough:
“哇,你要少吃木瓜,橙,萝卜,你的手掌很黄。。。你的肝功能不好,很容易发热生病。。。你开始有背酸了对不对,手上显示出来了。。。你的肠胃也不好。。。最近睡得不好是不是?“
and that's above my cough condition...
*sweats*
anw visited the chinese physician yest for my cough:
“哇,你要少吃木瓜,橙,萝卜,你的手掌很黄。。。你的肝功能不好,很容易发热生病。。。你开始有背酸了对不对,手上显示出来了。。。你的肠胃也不好。。。最近睡得不好是不是?“
and that's above my cough condition...
*sweats*
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